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. 2021 Sep 29;49(19):11167–11180. doi: 10.1093/nar/gkab840

Figure 3.

Figure 3.

miRNA adenylation requires F31C3.2/GLDR-2. (A) Heatmap summarizing percent of mono-adenylation across each miRNA (rows) in each RNAi condition (columns). Arrowhead above heatmap indicates column significantly different from vector, corresponding to F31C3.2 RNAi, which reduces adenylation. ***P-value = 0.0003 (B) Percent mono-adenylation in vector or F31C3.2/GLDR-2 RNAi. Three biological replicates of empty vector are shown in gray. (A, B) All miRNAs with >50 RPM in all empty vector replicates and an average of >1% mono-adenylation across the three empty vector replicates are shown. (C) Phylogenetic tree of F31C3.2/GLDR-2, GLD-2 and WISP. (Note that GLD2 in humans is also known as TENT2/TUT2/PAPD4/APD4). (D) Percent mono-adenylation in indicated genotypes/RNAi. Mean and standard error for four biological replicates are shown. MiRNAs shown are the same as those in (A, B).