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. 2021 Oct 6;49(19):11392–11404. doi: 10.1093/nar/gkab893

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Potential applications of SIBR. (A) OFF to ON switch by interrupting the translation of the GOI. (B) ON to OFF switch by degrading the POI after inducible attachment of a degradation tag. The degradation tag can be replaced by a localization tag as well. (C) Polycistronic operon control allowing constitutive expression of the first gene (Gene of interest 1; GOI 1) and inducible OFF to ON expression of the second gene (GOI 2). (D) Polycistronic operon control by allowing constitutive expression of both GOI 1 and GOI 2 and inducible protein degradation of GOI 2 upon induction. (E) A combination of all the potential SIBR applications in one single polycistronic operon.