Fig. 2. The same gelator forms gels with different mechanical properties on different surfaces. (a) Distribution of Young's modulus values (E) of hydrated 2-NapAV gels formed on an SP and an MC surface probed by nanoindentation (Optics11 system with a spherical tip radius of 22 μm). (b) The mean E of the hydrated gels on the SP and MC surfaces is not statistically significantly different (outliers removed using the ROUT method with Q = 1%, one tailed unpaired t-test, p = 0.2892, n = 2 for SP and n = 3 for MC). (c) The shear storage modulus (G^I, converted from E^I) of the gels prepared on SP and MC surfaces is not affected by surface chemistry (outliers removed using the ROUT method with Q = 1%, one tailed unpaired Mann–Whitney test, p = 0.2582, n = 1 for SP and n = 1 for MC), corroborating that hydrogels' elasticity is not modified by surface chemistry. (d) The shear loss modulus (G^II, converted from E^II) of the gels prepared on SP and MC surfaces is affected by surface chemistry (outliers removed using the ROUT method with Q = 1%, one tailed unpaired Mann–Whitney test, p = 0.0420, n = 1 for SP and n = 1 for MC). (e) The difference in G^II results in a statistically significant difference in tan(δ) = G^II/G^I (outliers removed using the ROUT method with Q = 1%, one tailed unpaired Mann–Whitney test, p = 0.0454, n = 1 for SP and n = 1 for MC).