Figure 7.

H2A and H3 protein expression is regulated by IL-33 in human glioma cells. Nuclear proteins and cytosolic proteins were isolated from Scramble- and IL33KD-U251MG cells (A). Additionally, the protein samples were prepared from MOCK- and IL33oe-U251MG cells (B). Western Blot analysis was conducted to measure the nuclear levels of H2A, H3, and Lamin A/C (as a loading control) in these transfectants. Representative images show the three proteins were only detected in the nuclear fraction. The nuclear levels of H2A and H3 in each immunoblot were quantified and normalized by the level of Lamin A/C. The results are presented as mean ± SEM from the four cell passages (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001 vs. Scramble in (A) or MOCK in (B). IL-33, interleukin-33.