Table 1.
Primer sequences for qPCR analysis and sequences for shRNA against IL-33.
| Rat IL-33 (NM_001014166) |
Forward: 5′-GTGCAGGAAAGGAAGACTCG-3′ Reverse: 5′-TGGCCTCACCATAAGAAAGG-3′ |
| Human IL-33 (NM_033439) |
Forward: 5′-TGGCCTACCATCCCTTCTGACCC-3′ Reverse: 5′-CGTTTTGGCATCCAGAGGTCAGGT-3 |
| Rat GAPDH (NM_017008) |
Forward: 5′-TCTACCCACGGCAAGTTC-3 Reverse: 5′-GATGTTAGCGGGATCTCG-3 |
| Human GAPDH (NM_002046) |
Forward: 5′-TTGGTATCGTGGAAGGACTCA-3 Reverse: 5′-TGTCATCATATTTGGCAGGTTT-3 |
| Rat BRCA1 (NM_012514.1) |
Forward: 5′-ACCACACAACTTAACAGGGCG-3 Reverse: 5′-TCAGGGTCTCTGCTGAGAACTA-3 |
| Rat Rad51 (NM_001109204.1) |
Forward: 5′-AGTGTGTTTGAGCCGTGTGA-3 Reverse: 5′-GCCATTACTGTCTCGCAGCC-3 |
| Human BRCA1 (NM_007294.4) |
Forward: 5′-agcagaatggtcaactgatgaata-3 Reverse: 5′-ACTGCTGCTTATAGGTTCAGCTTT-3′ |
| Human BRCA2 (NM_000059.4) |
Forward: 5′- aattagcatgtgagaccattgaga-3′ Reverse: 5′-gatttgtgtaacaagttgcaggac-3 |
| Human BRCC3 (NM_001025291) |
Forward: 5′- CGTTTGTCTCAACCACGCTC-3 Reverse: 5′-TTGTATCATCGTTCAACTCCCC-3′ |
| Human FANCB (NM_001018113.3) |
Forward: 5′- TGTTGTTGGAGTGAAAACTACAT-3′ Reverse: 5′- ACAAAGCTTTCCTCTTTCTTGCTA-3′ |
| Human FANCD (NM_033084.6) |
Forward: 5′- AGAGTTGTGGATGGCAAGGAC-3′ Reverse: 5′- AAGTTTGGGTCAAGTCGGAGG-3′ |
| Human Rad51 (NM_021770) |
Forward: 5′-CAGTGATGTCCTGGATAATGTAGC-3 Reverse: 5′-ttacc actgctacaccaaactcat-3 |
| Human HMGA1 (NM_145899.3) |
Forward: 5′- GCTGGTAGGGAGTCAGAAGGAG-3′ Reverse: 5′- GTCTTGGCAGCACCCTTGTT-3′ |
| Human HMGA2 (NM_003483.6) |
Forward: 5′- TGCCACCCACTACTCTGTCC-3′ Reverse: 5′- CCCTTGAAATGTTAGGCGGG-3′ |
| MOCK | pLVpuro-EF1a-F2A-GFP-Bsd |
| sh-rnoIL33_795 | AAAAGGCTCTGGTAGAAGAGAATTT GGATCCAAATTCTCTTCTACCAGAGCC |
| sh-hIL33_661 | AAAAGGAGTGTTTATAGGTGTAATTGG ATCCAATTACACCTATAAACACTCC |
| sh-scramble | AAAAGCTACACTATCGAGCAATTTTGGAT CCAAAATTGCTCGATAGTGTAGC |
| Full length IL-33 cDNA (NM_001014166) |
LIC-BamHI-rnoIL33-Forward 5′-3′ atagggcccgggttggatccgccaccatgagacctagaatgaagtattcgaactcc LIC-NheI-rnoIL33-Reverse 5′-3′ ggggagggagaggggctagcttacatcttagagagcttaaacatgatattgttac (pLV-EF1a-rnoIL33IRES-puro) |
| IL33-FLAG | pLVPuro-EF1a-rnoIL33-3xFlag |
IL-33, Interleukin-33.