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. 2021 Oct 20;12:711040. doi: 10.3389/fmicb.2021.711040

TABLE 2.

Effect of dietary treatmentsa on dry matter intake (DMI)b, live weight (LW)c, enteric emissionsd, and fermentation profilese in calves during three measurement phasesf.

P01
P02
P03
FO CO SEM P-T1 FO CO SEM HQ LQ SEM P-T1 P-T2 P-int FO CO SEM HQ LQ SEM P-T1 P-T2 P-int
DMI (kg/day) 0.89 2.1 0.039 < 0.01 3.50 3.31 0.202 3.98 2.83 0.202 0.52 < 0.01 0.81 4.11 3.91 0.168 4.20 3.81 0.168 0.4 0.12 0.87
LW (kg) 82.0 75.6 1.751 0.02 123.1 111.3 2.859 121.7 112.8 2.931 < 0.01 0.03 0.78 192.7 182.5 3.974 206.9 168.3 4.073 0.08 < 0.01 0.59
pCH4 (g/day)g 14.59 18.20 1.265 0.06 67.84 62.90 2.321 74.78 56.97 2.321 0.15 < 0.01 0.41 121.6 117.8 4.428 124.6 114.7 4.428 0.55 0.13 0.46
yCH4 (g/kg of DMI)h 16.21 8.66 0.690 < 0.01 20.60 19.42 1.412 18.87 21.16 1.412 0.56 0.27 0.91 29.81 30.41 0.901 29.91 30.32 0.901 0.65 0.75 0.68
pH2 (g/day)i 0.123 0.211 0.063 0.33 0.101 0.062 0.020 0.114 0.049 0.020 0.18 0.03 0.76 0.033 0.036 0.022 0.034 0.035 0.022 0.93 0.97 0.67
yH2 (g/kg)j 0.143 0.101 0.035 0.41 0.027 0.019 0.005 0.029 0.017 0.005 0.26 0.11 0.15 0.008 0.009 0.005 0.007 0.009 0.005 0.91 0.82 0.44
SCFA (mM)k 70.1 101.6 6.150 < 0.01 63.5 64.7 3.831 73.8 54.4 3.831 0.82 < 0.01 0.28 74.5 71.0 5.894 76.2 69.4 5.894 0.68 0.43 0.18
Acetate (%) 62.48 45.05 1.080 < 0.01 70.31 70.6 0.386 68.55 72.36 0.386 0.6 < 0.01 0.8 67.96 67.89 0.548 68.02 67.84 0.548 0.93 0.82 0.26
Propionate (%) 21.83 39.14 1.061 < 0.01 17.51 17.19 0.295 17.98 16.72 0.295 0.45 < 0.01 0.75 16.53 16.63 0.427 16.41 16.75 0.427 0.87 0.58 0.92
Butyrate (%) 12.04 10.99 0.801 0.37 9.19 8.95 0.255 9.41 8.73 0.255 0.52 0.07 0.71 11.71 11.64 0.276 11.77 11.58 0.276 0.87 0.65 0.14
Valerate (%) 1.35 3.53 0.179 < 0.01 0.94 1.00 0.039 1.20 0.74 0.039 0.32 < 0.01 0.32 0.99 1.02 0.038 0.99 1.02 0.038 0.49 0.67 0.43
Caproate (%) 0.45 0.68 0.097 0.11 0.29 0.34 0.027 0.28 0.35 0.027 0.17 0.07 0.13 0.12 0.13 0.008 0.13 0.11 0.008 0.43 0.17 0.72
Isobutyrate (%) 0.92 0.28 0.042 < 0.01 0.89 0.92 0.023 1.22 0.58 0.023 0.45 < 0.01 0.86 1.30 1.27 0.054 1.28 1.29 0.054 0.74 0.84 0.25
Isovalerate (%) 0.94 0.33 0.082 < 0.01 0.87 1.00 0.039 1.35 0.53 0.039 0.03 < 0.01 0.88 1.40 1.42 0.065 1.41 1.41 0.065 0.88 0.96 0.28

Results are the means and standard error of the means (SEM), P-value for treatment effect for FO vs. CO (P01), treatment effect for HQ vs. LQ (P02) and their interactions (P-int).

aDietary treatments corresponded to phase 1 (P01) concentrate (CO) vs. pasture (FO) diets and phase 2 (P02) high-quality (HQ) vs. low-quality (LQ) pastures, with measurements in P01 (9 weeks), P02 (19 weeks), and phase 3 (P03; 41 weeks) when all calves were offered a common pasture diet.

bDMI (kg/day) was measured in two consecutive days during gas emission measurements.

cLW (kg) was analyzed adjusting LW to initial LW.

dMethane (CH4) and hydrogen (H2) production in two consecutive days (g/day) and yield per kilogram of DMI (y; g/kg DMI) measured.

eTotal concentrations (mM) and individual proportions (%) of short-chain fatty acids (SCFA).

fDietary treatments in each phase were evaluated as follows: a one-way ANOVA in P01 (9 weeks) to analyze FO vs. CO diets and a 2 × 2 factorial ANOVA in P02 and P03 to evaluate FO vs. CO and HQ vs. LQ dietary treatment effects and their interactions.

gMethane production per animal (g of CH4/day).

hMethane yield (g of CH4/kg of DMI).

iHydrogen production per animal (g of H2/day).

jHydrogen yield (g of H2/kg of DMI).

kShort-chain fatty acids.