TABLE 2.
Effect of dietary treatmentsa on dry matter intake (DMI)b, live weight (LW)c, enteric emissionsd, and fermentation profilese in calves during three measurement phasesf.
| P01 |
P02 |
P03 |
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| FO | CO | SEM | P-T1 | FO | CO | SEM | HQ | LQ | SEM | P-T1 | P-T2 | P-int | FO | CO | SEM | HQ | LQ | SEM | P-T1 | P-T2 | P-int | |
| DMI (kg/day) | 0.89 | 2.1 | 0.039 | < 0.01 | 3.50 | 3.31 | 0.202 | 3.98 | 2.83 | 0.202 | 0.52 | < 0.01 | 0.81 | 4.11 | 3.91 | 0.168 | 4.20 | 3.81 | 0.168 | 0.4 | 0.12 | 0.87 |
| LW (kg) | 82.0 | 75.6 | 1.751 | 0.02 | 123.1 | 111.3 | 2.859 | 121.7 | 112.8 | 2.931 | < 0.01 | 0.03 | 0.78 | 192.7 | 182.5 | 3.974 | 206.9 | 168.3 | 4.073 | 0.08 | < 0.01 | 0.59 |
| pCH4 (g/day)g | 14.59 | 18.20 | 1.265 | 0.06 | 67.84 | 62.90 | 2.321 | 74.78 | 56.97 | 2.321 | 0.15 | < 0.01 | 0.41 | 121.6 | 117.8 | 4.428 | 124.6 | 114.7 | 4.428 | 0.55 | 0.13 | 0.46 |
| yCH4 (g/kg of DMI)h | 16.21 | 8.66 | 0.690 | < 0.01 | 20.60 | 19.42 | 1.412 | 18.87 | 21.16 | 1.412 | 0.56 | 0.27 | 0.91 | 29.81 | 30.41 | 0.901 | 29.91 | 30.32 | 0.901 | 0.65 | 0.75 | 0.68 |
| pH2 (g/day)i | 0.123 | 0.211 | 0.063 | 0.33 | 0.101 | 0.062 | 0.020 | 0.114 | 0.049 | 0.020 | 0.18 | 0.03 | 0.76 | 0.033 | 0.036 | 0.022 | 0.034 | 0.035 | 0.022 | 0.93 | 0.97 | 0.67 |
| yH2 (g/kg)j | 0.143 | 0.101 | 0.035 | 0.41 | 0.027 | 0.019 | 0.005 | 0.029 | 0.017 | 0.005 | 0.26 | 0.11 | 0.15 | 0.008 | 0.009 | 0.005 | 0.007 | 0.009 | 0.005 | 0.91 | 0.82 | 0.44 |
| SCFA (mM)k | 70.1 | 101.6 | 6.150 | < 0.01 | 63.5 | 64.7 | 3.831 | 73.8 | 54.4 | 3.831 | 0.82 | < 0.01 | 0.28 | 74.5 | 71.0 | 5.894 | 76.2 | 69.4 | 5.894 | 0.68 | 0.43 | 0.18 |
| Acetate (%) | 62.48 | 45.05 | 1.080 | < 0.01 | 70.31 | 70.6 | 0.386 | 68.55 | 72.36 | 0.386 | 0.6 | < 0.01 | 0.8 | 67.96 | 67.89 | 0.548 | 68.02 | 67.84 | 0.548 | 0.93 | 0.82 | 0.26 |
| Propionate (%) | 21.83 | 39.14 | 1.061 | < 0.01 | 17.51 | 17.19 | 0.295 | 17.98 | 16.72 | 0.295 | 0.45 | < 0.01 | 0.75 | 16.53 | 16.63 | 0.427 | 16.41 | 16.75 | 0.427 | 0.87 | 0.58 | 0.92 |
| Butyrate (%) | 12.04 | 10.99 | 0.801 | 0.37 | 9.19 | 8.95 | 0.255 | 9.41 | 8.73 | 0.255 | 0.52 | 0.07 | 0.71 | 11.71 | 11.64 | 0.276 | 11.77 | 11.58 | 0.276 | 0.87 | 0.65 | 0.14 |
| Valerate (%) | 1.35 | 3.53 | 0.179 | < 0.01 | 0.94 | 1.00 | 0.039 | 1.20 | 0.74 | 0.039 | 0.32 | < 0.01 | 0.32 | 0.99 | 1.02 | 0.038 | 0.99 | 1.02 | 0.038 | 0.49 | 0.67 | 0.43 |
| Caproate (%) | 0.45 | 0.68 | 0.097 | 0.11 | 0.29 | 0.34 | 0.027 | 0.28 | 0.35 | 0.027 | 0.17 | 0.07 | 0.13 | 0.12 | 0.13 | 0.008 | 0.13 | 0.11 | 0.008 | 0.43 | 0.17 | 0.72 |
| Isobutyrate (%) | 0.92 | 0.28 | 0.042 | < 0.01 | 0.89 | 0.92 | 0.023 | 1.22 | 0.58 | 0.023 | 0.45 | < 0.01 | 0.86 | 1.30 | 1.27 | 0.054 | 1.28 | 1.29 | 0.054 | 0.74 | 0.84 | 0.25 |
| Isovalerate (%) | 0.94 | 0.33 | 0.082 | < 0.01 | 0.87 | 1.00 | 0.039 | 1.35 | 0.53 | 0.039 | 0.03 | < 0.01 | 0.88 | 1.40 | 1.42 | 0.065 | 1.41 | 1.41 | 0.065 | 0.88 | 0.96 | 0.28 |
Results are the means and standard error of the means (SEM), P-value for treatment effect for FO vs. CO (P01), treatment effect for HQ vs. LQ (P02) and their interactions (P-int).
aDietary treatments corresponded to phase 1 (P01) concentrate (CO) vs. pasture (FO) diets and phase 2 (P02) high-quality (HQ) vs. low-quality (LQ) pastures, with measurements in P01 (9 weeks), P02 (19 weeks), and phase 3 (P03; 41 weeks) when all calves were offered a common pasture diet.
bDMI (kg/day) was measured in two consecutive days during gas emission measurements.
cLW (kg) was analyzed adjusting LW to initial LW.
dMethane (CH4) and hydrogen (H2) production in two consecutive days (g/day) and yield per kilogram of DMI (y; g/kg DMI) measured.
eTotal concentrations (mM) and individual proportions (%) of short-chain fatty acids (SCFA).
fDietary treatments in each phase were evaluated as follows: a one-way ANOVA in P01 (9 weeks) to analyze FO vs. CO diets and a 2 × 2 factorial ANOVA in P02 and P03 to evaluate FO vs. CO and HQ vs. LQ dietary treatment effects and their interactions.
gMethane production per animal (g of CH4/day).
hMethane yield (g of CH4/kg of DMI).
iHydrogen production per animal (g of H2/day).
jHydrogen yield (g of H2/kg of DMI).
kShort-chain fatty acids.