FIG. 7.

The formation of ternary complexes and functional synergy between EBF and E47 demands functional binding sites for both factors. (A) Autoradiograms from EMSAs with end-labeled wild-type or mutated 5′ NcoI-EcoRV fragments and 4 μl of in vitro-translated recombinant EBF and/or E47 as indicated. The gels displayed each represent one out of three experiments. F, free DNA. (B) The resulting luciferase activity when 200 ng of the indicated λ5 promoter reporter constructs was transiently cotransfected with 150 ng of EBF- and 50 ng of E47-encoding expression plasmids into HeLa cells. The reporter activity obtained with the wild-type promoter was set as 1, and data were collected from three representative transfections. Error bars indicate standard deviations.