Table 2. The real time RT-PCR results of TET1 and GAPDH genes.
Tissue samples | TET1 Ct | GAPDH Ct | GAPDH/TET1 ΔCt | copies TET1/10000 copies GAPDH 10000*(2^-ΔCt) | TET1 IHC score |
---|---|---|---|---|---|
EC-01 | 28.18 | 22.69 | 5.49 | 222.51 | 160 |
EC-02 | 32.94 | 25.15 | 7.79 | 45.18 | 20 |
EC-03 | 28.04 | 21.89 | 6.15 | 140.82 | 90 |
EC-04 | 28.63 | 21.34 | 7.29 | 63.90 | 30 |
EC-05 | 25.38 | 19.74 | 5.64 | 200.54 | 120 |
EC-06 | 25.35 | 18.85 | 6.5 | 110.49 | 60 |
EC-07 | 25.71 | 19.92 | 5.79 | 180.73 | 100 |
EC-08 | 24.62 | 18.32 | 6.3 | 126.91 | 90 |
EC-09 | 30.25 | 23.29 | 6.96 | 80.32 | 40 |
EC-10 | 28.59 | 20.84 | 7.75 | 46.45 | 20 |
EC-11 | 31.69 | 24.85 | 6.84 | 87.29 | 60 |
EC-12 | 32.85 | 24.32 | 8.53 | 27.05 | 10 |
EC-13 | 32.27 | 24.07 | 8.2 | 34.01 | 10 |
EC-14 | 29.1 | 21.91 | 7.19 | 68.48 | 30 |
EC-15 | 29.19 | 21.02 | 8.17 | 34.72 | 10 |
Normal-01 | 26.69 | 20.99 | 5.7 | 192.37 | 180 |
Normal-02 | 26.98 | 21.24 | 5.74 | 187.11 | 150 |
Normal-03 | 34.98 | 28.59 | 6.39 | 119.24 | 90 |
Normal-04 | 32.21 | 25.54 | 6.67 | 98.20 | 60 |
Normal-05 | 28.61 | 22.22 | 6.39 | 119.24 | 90 |
Normal-06 | 25.11 | 19.79 | 5.32 | 250.33 | 240 |
Normal-07 | 26.84 | 20.9 | 5.94 | 162.89 | 120 |
Normal-08 | 27.3 | 20.84 | 6.46 | 113.59 | 90 |
Normal-09 | 26.01 | 19.82 | 6.19 | 136.97 | 100 |
Normal-10 | 26.74 | 20.93 | 5.81 | 178.24 | 120 |
Normal-11 | 26.16 | 20 | 6.16 | 139.85 | 100 |
Normal-12 | 27.96 | 22.12 | 5.84 | 174.58 | 180 |
Normal-13 | 26.24 | 20.57 | 5.67 | 196.41 | 120 |
Normal-14 | 26.86 | 21.41 | 5.45 | 228.76 | 180 |
Normal-15 | 28.93 | 22.7 | 6.23 | 133.22 | 100 |