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. 2021 Oct 22;17(10):e1009807. doi: 10.1371/journal.ppat.1009807

Fig 3. Gp160ΔCT and SOS mutations consistently improve trimer expression.

Fig 3

A) VLP trimer expression with or without gp41 truncation (gp160ΔCT) and SOS mutations, probed with anti-gp120 and anti-gp41 MAb cocktail. SOS gp160ΔCT trimer expression of candidate strains visualized by B) BN-PAGE-Western blot and by SDS-PAGE-Western blot, probing with anti-gp120 (C) or anti-gp41 (D) MAb cocktails. All Envs were expressed using robust plasmids (pVRC8400 or pCDNA3.1), except for Q23 in part A lanes 6–8 and BB201 in part B lane 10, for which pCR3.1 was used. pVRC8400 was used to express Q23 in part B, lane 2.