Figure 2.

Infection of WT and global ELMO1KO mice with WT Salmonella (SL) and sifA mutant shows the involvement of ELMO1–SifA interaction in bacterial dissemination and inflammatory responses. (a) Schematic diagram represents the experimental design. (b) The percentage of weight loss was measured in WT and global ELMO1 KO mice infected via oral gavage with WT SL and sifA mutant strains for 5 days. (c-f) Bacterial burden was assessed at day 5 of infection in the cecum (c), spleen (d), liver (e), and ileum (f) of WT and global ELMO1 KO mice infected with WT SL and sifA mutant strains. (g-h) The H&E staining (g) and the pathology score (h) were assessed based on the degree of crypts loss, the infiltration of leukocytes in both mucosa and submucosa of WT and global ELMO1 KO mice infected with WT SL and sifA mutant. *, **, ***, and **** mean p ≤ 0.05, ≤ 0.01, ≤ 0.001, and ≤ 0.0001, respectively as assessed by Mann Whitney test