Figure 3.

Infection of WT and global ELMO1 KO mice with WT SL and sifA mutant shows the involvement of ELMO1–SifA interaction on the induction of innate immune responses. (a-e) Total RNA was isolated from the cecum of WT and global ELMO1 KO mice infected with WT SL and sifA mutant for 5 days as in Figure (2), the transcript level of inflammatory cytokines such as TNF-α (a), IL-6 (b), MCP-1 (c), IL-1β (d), and CXCL-1 (e) was measured by RT-qPCR. (f-j) Total RNA was isolated from the spleen of WT and global ELMO1 KO mice infected with WT SL and sifA mutant in Figure (2), the transcript level of inflammatory cytokines such as TNF-α (f), MCP-1 (g), IL-6 (h), IL-1β (i), and CXCL-1 (j) was measured by RT-qPCR. *, **, *** means p ≤ 0.05, ≤ 0.01, and 0.001, respectively as assessed by Mann Whitney test