Table 1.
Binding affinities of bemarituzumab to FGFR2b ECDs from 3 species
| Source of FGFR2b | Kon (1/Ms) | Koff (1/s) | KD (Koff/Kon) (nM) |
|---|---|---|---|
| Rat | 4.34E+04 | 2.65E-05 | 0.611 |
| Cynomolgus monkey | 4.39E+04 | 3.38E-05 | 0.770 |
| Human | 8.42E+04 | 4.88E-05 | 0.579 |
Kon = associate constant; Koff = dissociate constant; KD = equilibrium dissociation constant.
Surface plasmon resonance was used to determine binding affinities; 6 concentrations (0–500 nM) of FGFR2b ECD IgG fusion proteins were tested. Kon, Koff, and KD were calculated using the Biacore T100 Evaluation Software 1:1 binding model.