Figure 4.

Circ_0004018 sequesters miR-1197 and modulates PTEN expression to inactivate PI3K/AKT signaling pathway. (a) The protein levels of PTEN and related factors of PI3K/AKT pathway were analyzed by western blot after circ_0004018 was overexpressed. (b). The binding sequence of miR-1197 and PTEN was shown. (c). RIP assay exhibited the enrichment of circ_0004018, miR-1197, as well as PTEN in Ago2 group. (d). The binding between PTEN and miR-1197 was raveled out with the help of luciferase reporter assay. €. PTEN protein level was tested in cells with the transfection of different plasmids, including pcDNA3.1, pcDNA3.1-circ_0004018 and pcDNA3.1-circ_0004018+ miR-1197 mimics. **P < 0.01. n.s.: no significance