Table 1.
Sequences for RT-qPCR primers | |
---|---|
Gene | Primer sequence |
linear-SMYD4 | F GGGGTACCAGAGACCTGTCG R AGCAACCAGACGCACATTCT |
circ_0004018 | F AGCAGCAGTGACACCTGAAT R GACACGTCTGTGTGTTGTATGG |
PTEN | F CAGCAGCTTCTGCCATCTCT R TGCTTTGAATCCAAAAACCTTACT |
miR-1197 | Sequence: taggacacatggtctacttct Reverse transcription stem loop primer CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGC agaagt Stem loop universal reverse primer CTCAACTGGTGTCGTGGA F CCGAGtaggacacatggtct |
miR-378 g | Sequence: actgggcttggagtcagaag Reverse transcription stem loop primer CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGC cttctg Stem loop universal reverse primer CTCAACTGGTGTCGTGGA F TCGCAactgggcttggagt |
GAPDH | F GACAGTCAGCCGCATCTTCT R GCGCCCAATACGACCAAATC |
U6 | F TCCCTTCGGGGACATCCG R AATTTTGGACCATTTCTCGATTTGT |