Figure 4.

Co-transfection of miR-876-3p I and sh-circLNPEP reversed regulation of miR-876-3p I or sh-circLNPEP on proliferation of ovarian cancer cell lines

(a) miR-876-3p expression in four groups was determined by qRT-PCR. U6 was used as internal control. (b-c) The colony formations in four groups were determined by colony formation assay. Quantified values were expressed as mean ± standard deviation. Experiments were conducted independently in triplicate. *P < 0.05, **P < 0.01, ***P< 0.001 vs vector + IC group. ^{^^}P< 0.01, ^{^^^}P< 0.001 vs vector + I group. ^#P< 0.05, ^##P< 0.01, ^###P< 0.001 vs sh-circLNPEP + IC group. The experiments were divided into four groups: vector + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p IC), sh-circLNPEP + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPE sequences and miR-876-3p IC), vector + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p I) and sh-circLNPEP + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPEP sequences and miR-876-3p I).LNPEP: leucyl and cystinyl aminopeptidase. I: inhibitor. Sh: short hairpin. qRT-PCR: quantitative reverse transcription polymerase chain reaction. IC: inhibitor control.