Figure 5.

Co-transfection of miR-876-3p I and sh-circLNPEPreversed regulation of miR-876-3p I or sh-circLNPEPon migration and invasion of ovarian cancer cells, as well as angiogenesis of HUVECs

(a-b) Wound healing assay revealed the migration rate in four groups (magnification, 100×). Scale bar = 100 μm. (c-d) The invasion rates in four groups were determined by transwell assay (magnification, 200×). Scale bar = 100 μm. (e-f) Tube formation assay showed the tube length in four groups (magnification, 200×). Scale bar = 100 μm. Quantified values were expressed as mean ± standard deviation. Experiments were conducted independently in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs vector + IC group. ^{^}P < 0.05, ^{^^}P < 0.01, ^{^^^}P < 0.001 vs vector + I group.^###P < 0.001 vs sh-circLNPEP + IC group. The experiments were divided into four groups: vector + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p IC), sh-circLNPEP + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPEP sequences and miR-876-3p IC), vector + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p I) and sh-circLNPEP + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPEP sequences and miR-876-3p I).LNPEP: leucyl and cystinyl aminopeptidase. I: inhibitor. Sh: short hairpin. IC: inhibitor control. HUVECs: Human Umbilical Vein Endothelial Cells.