Figure 7.

Co-transfection of miR-876-3p I + sh-circLNPEP reversed the regulation of miR-876-3p I or sh-circLNPEP on expression of WNT5A

(a-b) The mRNA levels of c-Met, FOXA2, WNT5A and ZEB1 in four groups were determined by qRT-PCR. (c-d) The protein level of WNT5A in four groups was determined by western blot. Quantified values were expressed as mean ± standard deviation. Experiments were conducted independently in triplicate. *P < 0.05, ***P< 0.001 vs vector + IC group. ^{^^}P < 0.01, ^{^^^}P < 0.001 vs vector + I group. ^###P < 0.001 vs sh-circLNPEP + IC group. GAPDH was used as internal control. The experiments were divided into four groups: vector + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p IC), sh-circLNPEP + IC group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPEP sequences and miR-876-3p IC), vector + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector and miR-876-3p I) and sh-circLNPEP + I group (SKOV3 and SK-BR-3 cells were transfected with pGPU6 vector containing sh-circLNPEP sequences and miR-876-3p I).LNPEP: leucyl and cystinyl aminopeptidase. I: inhibitor. Sh: short hairpin. c-Met: mesenchymal-epithelial transition factor. FOXA2: Forkhead Box A2. ZEB1: Zinc-finger E-box binding protein 1. qRT-PCR: quantitative reverse transcription polymerase chain reaction. IC: inhibitor control. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.