Figure 6.

TrkB and miR-146a-3p targeting relationship verification, expression and correlation research in osteoarthritis (OA) cartilage tissues. (a) The TargetScan database predicted the targeted binding sites for TrkB and miR-146a-3p. (b-c) Dual-luciferase experiment was used to verify the targeting relationship between TrkB and miR-146a-3p. (d-e) The expression of miR-146a-3p and TrkB in OA cartilage tissues was detected by qRT-PCR. GAPDH served as an internal reference. (f-g) The correlation analysis between miR-146a-3p and TrkB in healthy human cartilage tissues and OA cartilage tissues was obtained by Spearman correlation coefficient. All experiments were repeated three times to average. *** p < 0.001 vs. miR-146a-3p mimic control (MC); ^## p < 0.01 vs. miR-146a-3p inhibitor control (IC); ^{^^^} p < 0.001 vs. Healthy