Figure 11.

Effect of silenced or overexpressed NEAT1 on miR-146a-3p expression and normal chondrocyte proliferation. (a) QRT-PCR was used to detect the transfection rate of silencing or overexpressed NEAT1 in normal chondrocytes. GABDH was served as an internal reference. (b) QRT-PCR was used to detect the effect of silenced or overexpressed NEAT1 on miR-146a-3p mRNA level. U6 served as an internal reference. (c) The effects of silencing overexpressed NEAT1 and miR-146a-3p mimic and inhibitor on the chondrocyte proliferation ability were detected by cell colony formation assay. All experiments were repeated three times to average. Normal chondrocytes: CHON-001. ^&& p < 0.01, ^&&& p < 0.001 vs. Empty vector; ** p < 0.01 vs. Empty vector + scramble; ^## p < 0.01 vs. NEAT1 + scramble; ^{^^} p < 0.01 vs. silenced NEAT1 (ShNEAT1) + scramble