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. 2021 Nov 3;7(45):eabe3954. doi: 10.1126/sciadv.abe3954

Fig. 4. Knockdown of ABI3 decreases migration of microglia in vitro.

Fig. 4.

(A) HMC3 microglia cells were transfected with ABI3-targeting or negative control siRNAs. ABI3 siRNA decreased mRNA level of ABI3 by 72% in HMC3 cells (n = 3 per group). (B) A scratch was made in the middle of the wells with WoundMaker tool. Cell confluency within the scratch area was analyzed 72 hours after using the IncuCyte S3. Yellow area shows initial scratch area, and green area shows the scratch area without cells. (C) Wound confluency was decreased in ABI3 knockdown group. (D) Relative wound density was decreased in ABI3-silenced microglia (n = 5 per group). Data represent means ± SEM. Unpaired t test; **P < 0.01 and ***P < 0.001.