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. 2021 Oct 27;2021:9416794. doi: 10.1155/2021/9416794

Table 1.

Primer sequences of RT-qPCR assay.

Gene name Sequence
Cu/Zn SOD Forward: 5′-AACCAGTTGTGTTGTCAGGAC-3′
Reverse: 5′-CCACCATGTTTCTTAGAGTGAGG-3′

Mn SOD Forward: 5′-CAGACCTGCCTTACGACTATGG-3′
Reverse: 5′-CTCGGTGGCGTTGAGATTGTT-3′

GSH-Px Forward: 5′-GTGCAATCAGTTCGGACACCA-3′
Reverse: 5′-CACCAGGTCGGACGTACTTG-3′

CAT Forward: 5′-GGAGGCGGGAACCCAATAG-3′
Reverse: 5′-GTGTGCCATCTCGTCAGTGAA-3′

IL-1β Forward: 5′-GAAATGCCACCTTTTGACAGTG-3′
Reverse: 5′-TGGATGCTCTCATCAGGACAG-3′

IL-12 Forward: 5′-CCTCCACTGTGCTGGTTTTAT-3′
Reverse: 5′-TCAGCAACATGCTCCAGAAG-3′

TNF-α Forward: 5′-CAGGCGGTGCCTATGTCTC-3′
Reverse: 5′-CGATCACCCCGAAGTTCAGTAG-3′

IL-10 Forward: 5′-CCAAGCCTTATCGGAAATGA-3′
Reverse: 5′-TTTTCACAGGGGAGAAATCG-3′

COX-2 Forward: 5′-TTCCAATCCATGTCAAAACCGT-3′
Reverse: 5′-AGTCCGGGTACAGTCACACTT-3′

iNOS Forward: 5′-GTTCTCAGCCCAACAATACAAGA-3′
Reverse: 5′-GTGGACGGGTCGATGTCAC-3′

NF-κB p65 Forward: 5′-TGCGATTCCGCTATAAATGCG-3′
Reverse: 5′-ACAAGTTCATGTGGATGAGGC-3′

IκB-α Forward: 5′-CGAGACTTTCGAGGAAATACCC-3′
Reverse: 5′-GTCTGCGTCAAGACTGCTACA-3′

β-Actin Forward: 5′-ATGGAGCCGGACAGAAAAGC-3′
Reverse: 5′-TGGGAGGTGTCAACATCTTCTT-3′

Cu/Zn SOD: cuprozinc-superoxide dismutase; Mn SOD: manganese superoxide dismutase; GSH-Px: glutathione peroxidase; CAT: catalase; IL-1β: interleukin 1β; IL-12: interleukin12; TNF-α: tumor necrosis factor-alpha; IL-10: interleukin 10; COX-2: cyclooxygenase-2; iNOS: inducible nitric oxide synthase; NF-κB p65: nuclear factor κ-light-chain-enhancer of activated B cells; IκB-α: nuclear factor of κ-light polypeptide gene enhancer in B-cell inhibitor-α.