Figure 4.

TUNEL assay results of A498 and ACHN cells treated with BCA (0, 10, and 20 μM) for 48 h. DNA fragmentation was shown by fluorescence microscopy. (a, c) Blue fluorescence represents the nuclei stained using Hoechst 33342, and green fluorescence indicates the TUNEL-positive cells. (b, d) The bar graph showed that the percentages of TUNEL-positive cells after BCA treatment. The error bar represented the standard error. Significant differences between BCA treat and DMSO control groups are indicated as ^∗ (^∗ means p < 0.05).