Figure 6.

BCA induced the nuclear localization of FOXO3 protein and BCA-mediated apoptosis was dependent on FOXO3. Nuclear fraction western blot analysis results of (a) A498 and (b) ACHN cells treated with BCA (0, 10, 20, and 40 μM) for 24 h. The expression levels of p27 and FOXO3 proteins in the cytoplasm and nucleus were investigated by performing western blot analysis. GAPDH, and Lamin A/C proteins were used for a gel-loading control for cytoplasmic and nuclear protein fractions, respectively. FOXO3 dependent apoptosis in renal cancer cells treated with BCA. Western blot results of (a) A498 and (b) ACHN cells transfected with control or FOXO3 siRNA and treated with BCA (0 and 10 μM) for 24 h. β-actin was used as a loading control protein.