Figure 4.
Malondialdehyde and total antioxidant levels in whole mouse brains. (a, c) Standard reference samples (MDA or uric acid) were serially diluted in certain ranges. Standard curves are presented with equations and R2 values. (b, d) Mouse whole brain samples incubated with the proper reagents were read at 532 nm (MDA) or 490 nm (antioxidants) for the calibration of quantity, and values were calculated according to the equations of the standard curves (n = 7 in sham and BCAO groups, n = 8 in H2 group). MDA: malondialdehyde; BCAO: bilateral common carotid artery occlusion; TBARS: thiobarbituric acid reactive substance; TAC: total antioxidant capacity; ∗p < 0.05.