Table 2.
Sequences for real-time PCR primers.
| Genes | Primer sequence (5′–3′) | Accession no. |
|---|---|---|
| β-Actin | F: GAGAAATTGTGCGTGACATCA R: CCTGAACCTCTCATTGCC |
L08165 |
| Occludin | F: ACGGCAGCACCTACCTCAA R: GGGCGAAGAAGCAGATGAG |
D21837.1 |
| ZO-1 | F: CTTCAGGTGTTTCTCTTCCTCCTC R: CTGTGGTTTCATGGCTGGATC |
XM_413773 |
| TRL4 | F: GTTCCTGCTGAAATCCCAAA R: TATGGATGTGGCACCTTGAA |
NM_001030693 |
| IL-1β | F: ACTGGGCATCAAGGGCTA R: GGTAGAAGATGAAGCGGGTC |
NM_204524 |
| TNF-α | F: GAGCGTTGACTTGGCTGTC R: AAGCAACAACCAGCTATGCAC |
NM_204267 |
| IFN-γ | F: TAACTCAAGTGGCATAGATGTGGAAG R: GACGCTTATGTTGTTGCTGATGG |
NM_008337 |
1Abbreviation: ZO-1: zonula occludens-1; TRL4: Toll-like receptor-4; IL-1β: interleukin-1β; TNF-α: tumor necrosis factor-α; IFN-γ: interferon-γ.