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. 2021 Jul 27;187(3):1728–1745. doi: 10.1093/plphys/kiab329

Figure 3.

Figure 3

Fe acquisition processes are enhanced in IMA1 and IMA2 overexpression lines. A, Rhizosphere acidification by 8-day-old plants grown on ES media after transferring to plates containing the pH indicator bromocresol purple for 24 h. A representative image of three independent experiments is shown. Acidifcation is indicated by yellow color around the roots. B, Ferric reductase activity of 12-day-old plants grown on either ES (Control) or navFe media for 3 days, after 9 days of precultivation on ES media. C, Level of IRT1 protein (∼34 kDa) in root samples from 12-day-old plants grown on either ES or navFe media for 3 days, after 9 days of precultivation on ES media. Coomassie blue (CB) was used as loading control. Per lane, 10 µg of total protein was loaded. An image representative of three independent experiments is shown. D, Expression of major Fe uptake genes in roots of 12-day-old seedling grown on ES or navFe media for 3 days, after 9 days of precultivation on ES media. Gene expression was determined by RT-qPCR using ΔCT method and expression of elongation factor 1 alpha as an internal control. Each bar in (B) and (D) represents the mean ± se of four independent experiments. Distinct letters above the bar graph indicate significant differences (P < 0.05) in two-way ANOVA followed by Tukey’s HSD test.