Figure 2.

CAH4/5 protein expression is under the control of CIA5. The left panel is an immunoblot probed with anti-CAH4 antibodies showing CAH4/5 levels in cia5 grown on MIN media in light at high CO₂ or ambient CO₂ compared to D66 grown at ambient CO₂. Cells were grown in MIN media for 72 h in high CO₂ conditions before incubating them under the respective CO₂ conditions. The right panel is an SDS–PAGE gel of the same samples stained with Coomassie Blue.