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. 2021 Aug 2;187(3):1619–1635. doi: 10.1093/plphys/kiab377

Figure 3.

Figure 3

Knockout of CsHEC2 led to reduced endogenous CTK content and disturbed expression of CTK pathway genes. A–C, The levels of endogenous CTK, (A) IPA, (B) ZR, and (C) DHZR were measured in WT and Cshec2 plants. Values are means ± SD of three independent biological replications. Significance analysis compared to WT was performed with the two-tailed Student’s t test (**P < 0.01, *P < 0.05, ns, no significant difference). D, Schematic diagram of a simplified biosynthesis and metabolic pathway for CTK. E, Heat map of the CTK pathway and related DEGs in WT and Cshec2 lines. Three biological replicates were performed and the colored bar on the left of the map represents fold change (log2 value). F–H, Representative morphology of cucumber ovaries at anthesis (F), magnified view of ovary at anthesis (G) and fruit wart density quantification (H) of WT and Cshec2 plants treated with 100 μM BAP. Values are means ± SD (n ≥ 15). The different lowercase letters indicate significant differences (P < 0.05) by one-way ANOVA analysis with Tukey’s HSD test. Scale bar: (F) 2 cm, (G) 1 mm.