Figure 3.

GsMTx4 inhibits the stretch-activated channel activity and the folding response of leaflets. A, Typical patch-clamp recording of proximal cells in outside-out configuration in control condition, in the presence of GsMTx4 (10 µM), then after wash-out and B, their corresponding mean (± sem) open probability (NPo). C–E, Application of GsMTx4 to the pulvinule inhibits the folding of the leaflet in response to mechanical stimulation in the different secondary leaflet zones with (C) 0.04-g, (D) 0.4-g, and (E) 2-g von Frey filament. Bars represent mean ± sem, n = 8. A paired t test was used to compare means (*P < 0.05).