Table 5.
Thioacidolysis of TMSD-methylated poplar CWs from BdPMT1-OE lines obtained in the WT and AtF5H-OE backgrounds, as compared to their respective controls
| Line | S/G Molar Ratio | % Free Phenolic Units in β-O-4 Linked G or S Units |
|
|---|---|---|---|
| (3 + 4)/(1 + 2) | %GOH | %SOH | |
| 100 × 1/(1 + 2) | 100 × 3/(3 + 4) | ||
| WT control | 2.05 (0.03)b | 19.45 (0.22)d | 2.81 (0.07)e |
| BdPMT1-OE/WT line 9 | 2.09 (0.18)b | 22.85 (0.12)b | 3.65 (0.19)bc |
| BdPMT1-OE/WT line 17 | 2.12 (0.19)b | 23.65 (0.48)a | 4.44 (0.25)a |
| BdPMT1-OE/WT line 31 | 2.08 (0.06)b | 21.09 (0.26)c | 3.44 (0.10)cd |
| AtF5H-OE control | 3.12 (0.13)a | 20.85 (0.44)c | 3.26 (0.03)d |
| BdPMT1-OE/AtF5H-OE line 21 | 2.96 (0.15)a | 23.10 (0.49)b | 4.02 (0.21)bc |
The S/G molar ratio corresponds to the ratio of the S monomers (3 + 4) to the G monomers (1 + 2; monomers shown in Figure 3). The molar % of free phenolic groups in β-O-4 linked G or S units, referred to as %GOH or %SOH, is calculated according to the outlined formula. The data represent mean (sd) values from biological triplicates. Different letters in columns indicate significant differences (Duncan test, P < 0.01).