Table 6.
Impact of a mild alkaline treatment (aq. NaOH 1 M, overnight, room temperature) on extract-free poplar stems from control and BdPMT1-OE lines obtained in the WT and AtF5H-OE backgrounds
| Line | %SR | %ABL in SR | %Alk-L |
|---|---|---|---|
| WT control | 68.03 (0.88)a | 23.69 (0.04)a | 15.5 (2.1)d |
| BdPMT1-OE/WT line 9 | 67.17 (0.46)a | 20.85 (0.47)b | 25.5 (1.1)a,b |
| BdPMT1-OE/WT line 17 | 65.25 (0.73)b | 21.52 (0.24)a,b | 28.1 (1.7)a |
| BdPMT1-OE/WT line 31 | 68.17 (0.22)a | 22.11 (0.75)a,b | 21.7 (1.6)b,c |
| AtF5H-OE control | 69.02 (0.65)a | 22.96 (0.28)a,b | 20.5 (1.3)c |
| BdPMT1-OE/AtF5H-OE line 21 | 67.65 (1.28)a | 21.52 (0.13)a,b | 26.4 (1.7)a |
The percentage of the recovered saponified residue (%SR) is expressed relative to the initial sample. The lignin content of the SR sample is measured as acetyl bromide lignin (%ABL). The percentage of alkali-soluble lignins (%Alk-L) is calculated from the ABL content of the CW and from the %SR recovery yield. The data represent mean (sd) values from biological triplicates. Different letters in columns indicate significant differences (Duncan test, P < 0.01).