Table 7.
Saccharification of the poplar SR obtained after a mild alkaline treatment (aq. NaOH 1 M, overnight, room temperature) and corresponding to BdPMT1-OE lines obtained in the WT and AtF5H-OE backgrounds, as compared to their respective controls
| SR from Line | %WL | Glc | Glc |
|---|---|---|---|
| mg·g−-1 SR | mg·g−-1 CW | ||
| WT control | 39.8 (1.3)d | 307.7 (16.0)e | 210.1 (11.3)e |
| BdPMT1-OE/WT line 9 | 52.1 (1.6)a,b | 417.5 (23.2)b,c | 280.1 (17.4)b,c |
| BdPMT1-OE/WT line 17 | 55.1 (2.0)a | 452.4 (17.7)a,b | 294.2 (8.3)a,b |
| BdPMT1-OE/WT line 31 | 45.8 (0.3)c,d | 369.3 (17.9)d | 251.8 (11.9)d |
| AtF5H-OE control | 44.2 (2.2)c,d | 401.4 (6.7)c,d | 277.1 (6.6)c,d |
| BdPMT1-OE/AtF5H-OE line 21 | 49.4 (1.5)b,c | 461.7 (22.4)a | 312.4 (16.5)a |
The saccharification efficiency is evaluated both by the weight loss (%WL) and by the released glucose (Glc). Glc yields are expressed either relative to the SR samples or to the initial CW samples. The data represent mean (sd) values from biological triplicates. Different letters in columns indicate significant differences (Duncan test, P < 0.01).