Figure 2.
Effect of TrxA levels on the FTR-Trx system proteins in the STXA2 strain. A, Western blot analysis of TrxA, TrxQ, TrxB, FTRC, and FTRV before (0 h) and after inducer removal (24–72 h). Samples equivalents to 4 ×107 cells of WT and STXA2 were taken at the indicated times and total proteins were isolated and resolved on SDS–PAGE, blotted and incubated with specific antibodies indicated in Supplemental Table S2. B, Quantification of the different proteins in the STXA2 strain before inducer removal (0 h) relative to the WT (dashed line). **P < 0.01 (two-tailed Student’s t test between WT and STXA2 strains). C, Quantification of the indicated proteins in the STXA2 strain after 48 h without inducer relative to the signal with inducer (0 h; dashed line). Quantifications were made using ImageJ software. Data are means ± sd from three biological replicates in all cases. *P < 0.05 and **P < 0.01 (two-tailed Student’s t test between 0 and 48 h).