Figure 4.

Effects of TrxA levels on photosynthetic-related proteins in the STXA2 strain. A, Western blot analysis of representative components of photosynthetic electron transport, ATP synthase, and chlorophyll synthesis in WT and STXA2 strains. Samples equivalents to 4 ×10⁷ cells were taken before (0 h) and after inducer removal (24–72 h). Total proteins were isolated and resolved on SDS–PAGE, blotted and incubated with specific antibodies. B, Quantification of the indicated proteins in the STXA2 strain before inducer removal (0 h) relative to the WT (dashed line). *P < 0.05 and **P < 0.01 (two-tailed Student’s t test between WT and STXA2 strains). C, Quantification of the indicated proteins in the STXA2 strain after 48 h without inducer relative to the signal with inducer (0 h; dashed line). Quantifications were made using ImageJ software. Data are means ± sd from three biological replicates in all cases. *P < 0.05 and **P < 0.01 (two-tailed Student’s t test between 0 and 48 h).