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. 2021 Jul 21;187(3):1414–1427. doi: 10.1093/plphys/kiab335

Figure 1.

Figure 1

Double mutant of NRP1 and NRP2 exhibits reduced tolerance to the ER stress inducer TM. A and B, TM sensitivity of Col, nrp1, nrp2, and nrpD. C and D, TM sensitivity of the complementation transgenic lines nrpD pNRP1:NRP1-mVenus (NRP1COM #2 and #7) and nrpD pNRP2:NRP2-mVenus (NRP2COM #1 and #9). Seven-day-old seedlings were transferred to 1/2 MS medium containing 1 μg/mL TM (DMSO as mock) for 10 d, and the picture was taken (A and C). The percentages of green-big (G-B), G-S, and Y-D seedlings were calculated (B and D). The images next to the boxes display the phenotype of plants in the three groups. Data represent means ± standard deviation (sd) of biological triplicates. Asterisks indicate significant differences compared with Col (Student’s t test, *P < 0.01; **P < 0.001).