Figure 1.

Experimental design, high throughput sampling, and color analysis. A, Total of 133 field-grown parents were randomly mated by pollinators, seeds were collected from each maternal plant, sown in progeny family blocks and grown for 5–6 weeks in a greenhouse. B, One fully expanded leaf was harvested and scanned from each plant in the population. C, Color thresholding was used to isolate binary masks for each leaf. Discrete RGB color matrices were converted to the continuous Lab color space, and color matrices for each leaf were spatially separated into segments: “full”—defined as the entire color matrix, “border”—defined as the outer 15% of pixels from the leaf boundary to the centroid, and “center”—defined as the inner 75% of pixels from the centroid to the boundary.