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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Methods. 2021 May 4;203:335–341. doi: 10.1016/j.ymeth.2021.04.024

Figure 3. Multiplex quantification of 5′-tRNA halves using cellular total RNA.

Figure 3.

(A) Schematic representation of multiplex quantification of 5′-tRNA halves using cellular total RNA.

(B) The total RNA from HeLa cells treated with SA or water (control: Ctrl) for 2 h were subjected to multiplex TaqMan RT-qPCR for the indicated 5′-tRNA halves. The quantified 5′-tRNA half levels were normalized to the levels of 5S rRNA. The amounts from control cells were set as 1, and relative amounts are indicated. Averages of three experiments with SD values are shown.