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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Methods. 2021 May 4;203:335–341. doi: 10.1016/j.ymeth.2021.04.024

Figure 4. Multiplex quantification of 5′-tRNA halves using plasma RNA.

Figure 4.

(A) Schematic representation of the convenient version of multiplex 5′-tRNA half quantification using plasma RNA.

(B) RNAs isolated from plasma samples of healthy individuals or Mtb-infected patients were subjected to the convenient version of multiplex TaqMan RT-qPCR for the indicated 5′-tRNA halves. The quantified 5′-tRNA half levels were normalized to the levels of spike-in RNA. The amounts from one of the healthy individuals were set as 1, and relative amounts are indicated. Averages of three experiments with SD values are shown.