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. 2021 Oct 21;12:750229. doi: 10.3389/fimmu.2021.750229

Table 1.

Primers and probes used in this study.

Oligonucleotide Sequence Type Annealing Temperature Label
E_Sarbeco_F ACAGGTACGTTAATAGTTAATAGCGT forward 60°C
E_Sarbeco_R ATATTGCAGCAGTACGCACACA reverse 60°C
E_Sarbeco_P1 ACACTAGCCATCCTTACTGCGCTTCG probe 60°C FAM-BHQ1
EAV-2043F CTGTCGCTTGTGCTCAATTTAC forward 60°C
EAV-2193R AGCGTCCGAAGCATCTC reverse 60°C
EAV 2102P-2 TGCAGCTTATGTTCCTTGCACTGTGTTC probe 60°C TXR-BHQ2
Leader_Sars2 CCCAGGTAACAAACCAACCAAC forward 60°C
Cyclophilin_A_F GGTGGTAAGTCCATCTACGG forward 54°C
Cyclophilin_A_R ACTCTGAGATCCAGCTAGGC reverse 54°C
ACE2_F TTGTATCTGTTGGCCTTCCC forward 53°C
ACE2_R TCTTGATCCTGAAGTCACGC reverse 53°C
TMPRSS2_F TGGGTTGAGTCAAATCTGCC forward 55°C
TMPRSS2_R CTACAGTTTACCTGCTGGCC reverse 55°C
Beta-actin_F TGACCGGATGCAGAAGGA (38) forward 51°C
Beta-actin_R CCGATCCACACCGAGTACTT (38) reverse 51°C