Fig. 5.
Molecular (contrast-enhanced) MSOT imaging at the whole-brain level. a 4T1 cells (0.7 × 106 injected intracranially) stably expressing ReBphP-PCM imaged using MSOT at a depth of 3.6 mm in the brain (arrow I) immediately after injection. Adapted with permission from [71]; b volumetric MSOT imaging of amyloid-beta plaque in Alzheimer arcAβ mouse brain using amyloid binding probe CRANAD-2, showing higher signal retention in the cortical areas compared to age-matched wild-type mice. Adapted with permission from [120]; c-f whole-brain functional optoacoustic neuro-tomography (FONT) volumetric imaging of calcium waves induced by pentylenetetrazole (PTZ) injection into an isolated mouse brain model. Adapted with permission from [31]; c absorption spectrum of purified calcium-saturated (blue) and calcium-free (red) GCaMP6f proteins. d Time traces of the normalized FONT data. Gray traces represent tetrodotoxin (TTX) injected 180 s before PTZ (t = 0 s), abolishing the activation; e no signal change due to PTZ injection are detected in a control isolated CD-1 mouse brain not expressing GCaMP6f proteins; f temporal evolution of the relative signal changes (∆OA/OA0) in slices at depths of 0.7 mm and 1.1 mm in a GCaMP6f-expressing brain