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. 2021 Nov 3;12:6353. doi: 10.1038/s41467-021-26461-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a The heatmap shows mean on-target activity of three parallel transfections normalised to the active splice donor site plasmid. A set of 34 previously used targets (Fig. 5) were used to determine on-target editing efficiencies of dABE8e, nABE8e and 6 increased fidelity ABE8e base editors. b Mismatch tolerance of ABE8e and its increased fidelity variants were compared utilising the exact same matching sgRNA (target 1 in Fig. 5) and 50 sgRNAs mismatching in one, two, three, four or five positions as indicated as red letters. Blue and yellow heatmaps show the mean normalised activity (off-target/on-target) derived from three parallel transfections. White and red heatmaps show the mean on-target activity (percentages of GFP positive cells) derived from three parallel transfections. For source data see the Source Data file.