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. 2021 Nov 3;7:331. doi: 10.1038/s41420-021-00663-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A t-SNE plot showing the 6 malignant cell subclusters from 5 PDAC samples. B Six malignant PDAC cell subclusters were identified by t-SNE analysis. C Violin plots for marker genes of cancer stem cells (ALDH1A1, PROM1, and NES) and ductal cells (MMP7, TSPAN8, and SOX9). D Feature plots for marker genes of cancer stem cells (ALDH1A1, PROM1, and NES) and ductal cells (MMP7, TSPAN8, and SOX9). The color legend shows the log1p normalized expression levels of the genes. E Heatmap showing the representative pathway terms of Hallmark enriched in each cellular subgroup. F Heatmap showing the representative pathway terms of KEGG enriched in each cellular subgroup.