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. 2021 Nov 3;11:21583. doi: 10.1038/s41598-021-00570-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fertilization assays using increasing sperm concentrations (from 100 sperm to over 10 million sperm per milliliter). Eggs of a single female were used for sperm from each male (A–D) throughout these assays. Two independent cycles of experiments were performed and one cycle of the dataset is shown. Bindin #2 represents sperm from an adult whose embryos were injected with Cas9 and gRNAs, yet no mutations were detectable in this animal. (Male B2 in Table #1).