Table 2.

Tabulation of mutations and phenotypes.

gRNA	Male individuals mutated	Female individuals mutated
82	B6 mutation but does not change ORF	B1, 2, and have a frameshift mutation
325	B7	None
373	B1, B3, B4 (although B1 mutation does not shift ORF), B5, B8	B3 has a frameshift mutation
455	B1, B3, B5, B7	None

Overall: 10/11 animals microinjected with Cas9/gRNAs had mutations (91%), 2 of the mutations did not disrupt the open reading frame (20%), and 4 of the 10 had mutations at multiple gRNA sites (40%). The useful mutation rate was thus 73%.

*No Bindin refers to results of immunoblots and immunolocalization data in Wessel et al., 2021).