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. 2021 Nov 3;4:1258. doi: 10.1038/s42003-021-02793-9

Fig. 2. Delayed endochondral ossification and downregulation of Mmp13 expression in Zfhx4-deficient mice.

Fig. 2

a Hemolysin and eosin (HE)-stained femurs of E15.5 wild-type (WT) and Zfhx4−/− littermates. Scale bar = 500 μm. b Von Kossa-stained femurs of E16.5 WT and Zfhx4−/− littermates. Scale bar = 200 μm. c Expression of chondrogenic gene markers in Zfhx4−/− mice. Paraffin-embedded sections of femurs from E15.5 WT and Zfhx4−/− littermates were examined by in situ hybridization using anti-sense probes against Sox9, Col2a1, Ihh, Ppr, Runx2, Osterix, Col10a1, and Mmp13. Scale bar = 500 μm. d Immunofluorescence analysis of femurs from E15.5 WT and Zfhx4−/− littermates using anti-Col2, anti-Col10, and anti-Mmp13 antibodies. Scale bar = 500 μm. e mRNA expression levels of chondrocyte marker genes. mRNA isolated from humeri of E15.5 WT and Zfhx4−/− littermates was analyzed by RT-qPCR. Data are presented as the mean ± s.d. (WT: n = 5; KO: n = 3).