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. 2021 Nov 3;4:1258. doi: 10.1038/s42003-021-02793-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Physical interaction of Zfhx4 with Osterix. Coimmunoprecipitation analysis of lysates of 293FT cells transfected with Flag-Zfhx4, 6xMyc-Osterix, or both is shown. Closed arrows: 6xMyc-Osterix, Open arrow: Flag-Zfhx4 (IP immunoprecipitation, WB western blotting). b Colocalization of Flag-Zfhx4 (red) with Venus-Osterix(green) in SW1353 cells. Scale bar = 5 μm. c Skeletal preparations of whole bodies, forelimbs, and hindlimbs of E16.5 wild-type (WT), Zfhx4^+/−;Osterix^+/−, Zfhx4^−/−, and Zfhx4^−/−;Osterix^+/− littermates stained with Alcian blue and alizarin red. Scale bar = 1 mm. Right panels show higher magnification images of the forelimbs and hindlimbs shown in the left panel. d–f Paraffin-embedded sections of E16.5 WT, Osterix^+/−, Zfhx4^+/−;Osterix^+/−, Zfhx4^−/−, and Zfhx4^−/−;Osterix^+/− littermates subjected to hematoxylin and eosin (HE) staining (d), von Kossa staining (e), and immunofluorescence staining with an anti-Mmp13 antibody (f). Scale bar = 500 μm.