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. 2021 Nov 3;4:1258. doi: 10.1038/s42003-021-02793-9

Fig. 4. Critical role of Zfhx4 in palate development.

Fig. 4

a Zfhx4 expression in the palatal shelf of E13.5 mice determined by in situ hybridization. Scale bar = 200 μm. b Coronal sections of crania of E13.5, E14.5, and E16.5 wild-type (WT) and Zfhx4−/− littermates stained with hematoxylin and eosin (HE). Scale bar = 500 μm. c Fluorescence microscopy images of coronal sections of crania of E13.5 WT and Zfhx4−/− littermates subjected to immunofluorescence analysis using an anti-Pcna antibody. Scale bar = 100 μm. d Coronal sections of crania of E13.5 WT and Zfhx4−/− littermates subjected to immunofluorescence analysis using an anti-Ki67 antibody. Scale bar = 100 μm. e Expression levels of genes associated with palatal development in the palatal shelves of E13 WT and Zfhx4−/− littermates as determined by RT-qPCR using TaqMan probes. Data represent the mean ± s.d. (WT: n = 3; KO: n = 3). f Coronal sections of crania of E13.5 WT and Zfhx4−/− littermates were examined by in situ hybridization using anti-sense probes against Osr1, Osr2, and Pax9. Scale bar = 200 μm.