Fig. 1. Chd7 expression in wildtype and Atoh1^Cre/+;Chd7^f/f mutant organ of Corti.

a Immunohistochemistry in wildtype cochlea showing Chd7 expression in hair cells at E16.5 and P7. Expression is weaker at P7 compared to E16.5. Hair cells are stained with F-actin and Calbindin 1. Tectorial membrane is stained with Satb2. b In-situ hybridisation showing loss of Chd7 expression in Atoh1^Cre/+;Chd7^f/f mutant hair cells within the middle region of the cochlea at E16.5. Atoh1^Cre/+ or Chd7^floxed siblings were used as controls (indicated at the top of the panel). IHC inner hair cells; OHC outer hair cells; TM tectorial membrane. Scale bars 25 µm.