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. 2021 Nov 3;7:333. doi: 10.1038/s41420-021-00725-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Volcano map represents the differentially expressed genes upon piR-57125 overexpression in OSRC-2 cells from RNA-seq. The experiments were carried out three times with the p < 0.05, |log2FC| > 0. b, c Decreased or increased mRNA (b) or protein (c) expression of CCL3 in 786-O cells via transfection with piR-57125 inhibitors or mimics respectively. Data represent mean ± S.D. from three independent experiments. d ELISA experiment showed that the secretion of CCL3 proteins from ccRCC cells was respectively increased or decreased upon knockdown or overexpression of piR-57125. Data represent mean ± S.D. from three independent experiments. e RIP and RT-qPCR assays revealed that piR-57125 and CCL3 mRNA had more affinity with PIWIL4 compared with the IgG control. Data represent mean ± S.D. from three independent experiments. f Luciferase reported plasmids which contained WT or Mut 3’UTR region of CCL3 were combined with piR-57125 or control mimics, and transfected into HEK-293T cells. The relative luciferase activity was normalized with renilla luciferase. Data represent mean ± S.D. from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.