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. 2021 Nov 3;7:333. doi: 10.1038/s41420-021-00725-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Expression of CCL3 in ccRCC and normal patients based on analysis of TCGA data. b RT-qPCR analysis of CCL3 expression in 45 paired ccRCC and adjacent normal samples from patients recruited from the SRRSH cohort. c correlation analysis showed that piR-57125 was negatively correlated with CCL3 expression in ccRCC patients from the SRRSH cohort. d Western blot analyzed the knockdown efficiency of CCL3 siRNAs. e Transwell assay revealed that knockdown of CCL3 restrained 786-O and OSRC-2 cells metastasis. Data represent mean ± S.D. from three independent experiments. f Wound healing assay demonstrated that knockdown of CCL3 inhibited 786-O and OSRC-2 cells metastatic ability. Data represent mean ± S.D. from three independent experiments. g Transwell assay demonstrated that the metastatic ability of ccRCC cells was inhibited when rhCCL3 was added exogenously (30 ng/ml). Data represent mean ± S.D. from three independent experiments. h Wound healing assay illustrated that exogenously added rhCCL3 (30 mg/ml) promoted 786-O and OSRC-2 cells migration and invasion. Data represent mean ± S.D. from three independent experiments. **p < 0.01; ***p < 0.001.